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Test Code BPRPV Bordetella pertussis and Bordetella parapertussis, Molecular Detection, PCR, Varies

Additional Codes

Torrance Memorial Laboratories Test Code:  62871682


Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by polymerase chain reaction (PCR) requires the specimen to be processed in an environment in which contamination of the specimen by Bordetella pertussis or Bordetella parapertussis DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Nasopharyngeal swab

Supplies:

Culture Swab - Liquid Stuarts/Single Swab (NP Swab) (T515)

Container/Tube: Rayon swab with an aluminum shaft placed in transport medium such as a green-top nasopharyngeal swab (rayon mini-tip) with Stuart's media (no charcoal), or Stuart's media with charcoal, or Amies media with or without charcoal (Transwab Nasopharyngeal with Charcoal System).

Additional Information:

1. Swab transport containers without charcoal must contain a pledget saturated with either Stuart's or Amies liquid media. Clear semi-solid/solid media is gel and will be rejected.

2. Other swab or media types may be inhibitory to PCR testing and will be rejected.

 

Acceptable:

Specimen Type: Nasopharyngeal (not throat) aspirate/wash or nasal aspirate/wash

Container/Tube: Sterile container with a screw top cap (no transport media)

Specimen Volume: Entire collection


Useful For

Preferred diagnostic test for the detection of Bordetella pertussis or Bordetella parapertussis

 

This test is not recommended for screening asymptomatic individuals who may carry B pertussis or parapertussis.

 

This test is not recommended for follow up of patients previously diagnosed with pertussis (ie, as a test of cure).

Method Name

Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Reporting Name

Bordetella, PCR, Varies

Specimen Type

Varies

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Ambient  7 days
  Frozen  7 days

Reject Due To

Nose, nasal, or throat swab
Calcium alginate or cotton-tipped swab
Swab sent in gel transport medium, viral/universal transport medium, or Regan Lowe media
ESwab
Swabs with solid plastic shaft
Dry swab
Reject

Reference Values

Not applicable

Day(s) Performed

Monday through Friday, Sunday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798 x 2

87798 (if appropriate for government payers)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BPRPV Bordetella, PCR, Varies 90441-7

 

Result ID Test Result Name Result LOINC Value
BPRS Specimen source 31208-2
618312 Bordetella pertussis PCR 43913-3
618313 Bordetella parapertussis PCR 42588-4

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.