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Test Code 8066022635 Testosterone Total and Free with SHBG, Immunoassay

Important Note

Test performed at TMMC effective 9/15/2026

Performing Laboratory

Torrance Memorial Laboratories

Specimen Requirements

Submit the following specimen:
 

Specimen Type: Serum

Container/Tube: Serum gel

Additional Instructions:   Allow to sit for 30 minutes prior to spinning.  Spin within 2 hours of collection.

 

Specimen Transport Temperature

Refrigerated

 

Storage Temp Stability:
Refrigerated Temperature: 3 days
 

Methodology

Chemiluminescent Immunoassay Performed on the Abbott Alinity i

Reference Range

 

Category Testosterone Total (ng/dL) Testosterone Free, Calculated (pg/mL) SHBG (nmol/L)
 
 
Males      
(21-49 years of age) 253-803 47.0-173.0 16-68
Males      
( ≥ 50 years of age) 248-674 42.7-150.4 14-70
Females      
(Premenopausal, 21-49 years of age) 15-50 1.4-10.5 15-122
Females      
(Postmenopausal, ≥ 50 years of age) 13-34 1.2-6.7 17-124

 

Interpretive Data:

Free testosterone concentration is calculated using total testosterone (measured by immunoassay) and the binding constant of testosterone, albumin, and sex hormone-binding globulin (SHBG). Use to evaluate hypogonadism in cisgender males with a total testosterone concentration at the lower limit of normal.

Testosterone immunoassays are both imprecise and inaccurate at low testosterone concentrations, such as those found in children and cisgender females. Due to DHEA-S cross-reactivity with Testosterone, conditions with elevated DHEA-S such as polycystic ovarian syndrome (PCOS) and congenital adrenal hyperplasia could have falsely elevated testosterone values. For these individuals, testing by mass spectrometry is recommended.

No reference intervals have been established for males or females younger than 21 years of age.

 

 *Note: Reference ranges provided on this web site are for guidance only, and may not reflect the most recent changes. Refer to laboratory reports for current data.   

Day(s) Test Set up/TAT

Performed:

Daily

Available STAT

TAT:

Routine: < 4 hrs

Aliases

Testos Tot+Fr

CPT Code

84402

84403

84270